Â鶹AV Integrative Genomics (BIG) Unit

The new state-of-the-art, custom designed Â鶹AV Integrative Genomics (BIG) Unit is housed within the Moulsecoomb campus at the Â鶹AV. 

The facility was established with significant grants and investment from the Natural Environment Research Council (NERC), Biotechnology and Biological Sciences Research Council (BBSRC), the European Commission, Higher Education Funding Council for England (HEFCE) and the Â鶹AV. 

The equipment, expertise and services listed below are accessible to academics, individuals and industry. 

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Genomics Â鶹AVThe Â鶹AV Integrative Genomics Unit at the Â鶹AV

The Â鶹AV Integrative Genomics Unit is a self-contained facility run by , Director of the Centre for Precision Health and Translational Medicine, with Bioinformatics Experimental Officer, Dr Andrew Hesketh.

If you are interested in accessing any of the services detailed below, you can contact the team directly at Genomics@brighton.ac.uk or by completing the enquiry form.

Sequencing

 

Next Generation Sequencer (NGS): Illumina NextSeq500 sequencer

High Output kit (up to 400 million clusters)

Single end (custom, 75, 150)

Paired end (custom, 75, 150 bp)

Medium Output kit (up to 130 million clusters)

Single end (custom, 150 bp)

Paired end (custom, 75, 150 bp)
NextSeq 500 DNA sequencing system

Library preparation

Quality control of nucleic acids:

  • Labtech Nanodrop One C spectrophotometer
  • Agilent 4200 TapeStation
  • Qubit 2.0 fluorometer
  • AriaMx qPCR machine
  • Alpha cycler 4 PCR machine

DNA size selection:

  • Blue Pippin (Sage Science)
  • Covaris M220 focussed DNA ultra-sonicator 

Major equipment for nucleic acid QC and quantification 

Agilent 4200 TapeStation System

Instrument for the quality control of nucleic acids: integrity (RIN-equivalent scoring), sizing and approximate quantification.

Agilent 4200 Tapestation for QC

Quantitative real time PCR

Agilent AriaMx Real time PCR system for accurate library quantification.

Agilent AriaMx qPCR machine

Applications supported at Â鶹AV Genomics

DNA sequencing

  • De novo genome sequencing (bacterial, small genomes and large genomes)
  • Exome sequencing
  • Amplicon sequencing
  • ChIP sequencing
  • Methylome sequencing (collaborative projects only) 

DNA microarrays

  • Expression microarrays (including RNA isolation, RNA labelling, microarray hybridization, washing and scanning)

RNA-seq

  • Small RNA sequencing
  • Total RNA sequencing
  • Stranded mRNA sequencing

Ribosome profiling

  • Bacterial and eukaryotic systems (collaborative projects only)

Prices for sequencing only*

*(quality controlled normalised libraries to be provided by user). See Sample Preparation Guidelines (pdf).

To obtain specific pricing for preparation of samples and sequencing libraries for any of the above applications, please complete the enquiry form. 

Using NextSeq 500 sequencing kits:

75 cycles up to 400 million clusters

1 x 75 bp (or less); 2 x 33 bp PE

£1,557

150 cycles up to 400 million clusters

1 x 150 bp; 2 x 75 bp PE

£2,713

300 cycles up to 400 million clusters

2 x 150 bp PE

£4,153

150 cycles up to 130 million clusters

1 x 150 bp; 2 x 75 bp PE

£1,182

300 cycles up to 130 million clusters

2 x 150 bp PE

£1,765

Equipment for hire on site

Name

Manufacturer

Description

Next Seq 500

Illumina

Next Generation Sequencer

Microarray Scanner C

Agilent Technologies

DNA microarray scanner at 2 μm resolution

Oven for DNA microarray hybridization

Agilent Technologies

Oven with rotisserie for the hybridization of DNA microarrays

AriaMx

Agilent Technologies

Real time PCR equipment

4200 TapeStation 

Agilent Technologies

Instrument for the quality control of nucleic acids

Nanodrop One C

LabTech International

Spectrophotometer for small volumes

PCR alpha cycler 4

LabTech

PCR multiblock

Vilber Lourmat Fusion FX Integrated imaging system

LabTech

Digital gel imaging system for detecting fluorescent, chemiluminescence and infrared signals

Tissuelyser

Qiagen

Cell disruption system

Blue Pippin

Sage Science

DNA size fractionation system

M220 Focussed ultra-sonicator

Covaris

Instrument for the controlled shearing of DNA for NGS

Qubit 2.0 Fluorometer

Thermo Fisher Scientific

Fluorometer for nucleic acid quantification

Additional equipment 

Name

 Manufacturer

 Description

Eppendorf refrigerated centrifuge 5430R+rotor FA-4

Thermo Fisher Scientific

Centrifuge for 1.5ml tubes

Eppendorf Thermomixer C

Thermo Fisher Scientific

Mixer/incubator for 1.5ml/0.2ml tubes

Eppendorf Concentrator F-45-48-11 GB 

Thermo Fisher Scientific

Vacuum concentrator

Labcaire chemical fume hood Aura 250/255 and 2x AC safety filters

Thermo Fisher Scientific

Chemical fume hood

Haier Upright Freezer, 728 litre capacity

Scientific Laboratory Supplies

-80°C freezer

Ice maker

Hoshizaki

Ice maker

Water purification system

ELGA

Water purification system up to 18.2 megohms grade